s1p d18  (Croda International Plc)

Journal: The FASEB Journal

Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis

doi: 10.1096/fj.202502924RR

Figure Lengend Snippet: ORMDLs reduction does not correlate with phosphorylated sphingolipid levels and is unaffected by direct dhS1P or S1P treatment. (A) Quantification of phosphorylated sphingolipids by LC‐ESI‐MS/MS in HEK293 cells treated with low or high concentrations of myriocin, FB 1 , or their combination, (B) together with relative quantification of ORMDLs, SPTLC1, SPTLC2, and phosphorylated AKT (S473) protein levels. (C) Analysis of ORMDLs, SPTLC1, SPTLC2, and the pAKT S473 /AKT ratio in HEK293 cells following a 6‐h desensitization period and subsequent 30‐ or 60‐min stimulation with dhS1P or S1P. In both (A) and (B), protein levels were normalized to total protein loading based on Ponceau S staining. (D) Representative time‐lapse images of HEK293 cells showing morphological responses to dhS1P or S1P stimulation. Scale bar: 100 μm. Data in (A‐C) are presented as geometric means ± GSEM ( N = 3). Statistical significance was assessed by one‐way ANOVA followed by Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.

Article Snippet: Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.

Techniques: Tandem Mass Spectroscopy, Quantitative Proteomics, Staining

Journal: The FASEB Journal

Article Title: ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell‐Type Dependent and Tied to Ceramide Homeostasis

doi: 10.1096/fj.202502924RR

Figure Lengend Snippet: Regulation of ORMDLs stability in human RPE‐1 cells and mouse BMMCs. (A) Levels of ORMDLs, SPTLC1, SPTLC2, and total d18:1 ceramides (measured by LC‐ESI‐MS/MS) were assessed in human RPE‐1 cells and mouse BMMCs after 24‐h treatment with 10 μM myriocin or 10 μM FB 1 , and compared with untreated controls. (B) Quantification of ORMDLs, SPTLC1, SPTLC2, and the LC3‐II/LC3‐I ratio was performed in untreated RPE‐1 cells and BMMCs, and compared with cells treated for 24 h with 50 μM CQ or 10 μM MG132, as determined by immunoblotting. (C) Effects of p97/VCP inhibition by CB‐5083 on ORMDLs, SPTLC1, SPTLC2, the LC3‐II/LC3‐I ratio, ATF4, and p62 levels were assessed in RPE‐1 cells and BMMCs. Protein levels in (A‐C) were normalized to total protein loading using Ponceau S staining. Data are presented as geometric mean ± GSEM ( N = 4). Statistical analysis was performed using one‐way ANOVA with Tukey's post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****); n.s., not significant.

Article Snippet: Sphingolipid concentrations of S1P, dhC1P, and C1P were determined by single‐point calibration using external standards [ ]: 50 ng S1P d18:1 (Cat# 860492), 50 ng dhC1P d18:0/C16:0 (Cat# 860522), and 50 ng C1P d18:1/C16:0 (Cat# 860533), all from Avanti Polar Lipids.

Techniques: Tandem Mass Spectroscopy, Western Blot, Inhibition, Staining

Journal: Cardiovascular diabetology

Article Title: Inverse relationship between circulating sphingosine-1-phosphate and precursor species and coronary artery calcification score in type 2 diabetes.

doi: 10.1186/s12933-025-02624-9

Figure Lengend Snippet: Fig. 1 Biosynthesis pathways of sphingosine-1-phosphate species. Major biosynthesis pathways of Sphingosine-1-Phosphate species (d16:1-S1P, d18:0- S1P, d18:1-S1P and d18:2-S1P) and their precursors (dihydrosphingosine and sphingosine) (in red). Dihydrosphingosine (Sphinganine); S1P, Sphingosine- 1-Phosphate; SphK, sphingosine kinase; SPT, Serine palmitoyl transferase

Article Snippet: S1P and internal standard were detected using multiple reaction monitoring (m/z 380 → 264 and 366 → 250, respectively), and were quantified using a seven-point calibration curve with d18:1-S1P as external standard (#860,492, Avanti Polar Lipids).

Techniques:

Journal: Cardiovascular diabetology

Article Title: Inverse relationship between circulating sphingosine-1-phosphate and precursor species and coronary artery calcification score in type 2 diabetes.

doi: 10.1186/s12933-025-02624-9

Figure Lengend Snippet: Fig. 2 Correlation between sphingosine-1-phosphate and precursors species with cholesterol in high-density lipoproteins. Correlation between HDL S1P species, d18:2-S1P panel A, d18:1-S1P panel B, d16:1-S1P panel C, d18:0-S1P (panel F) or precursor, sphingosine panel D, dihydrosphingosine panel E and plasma HDL-cholesterol levels in patients with type 2 diabetes (n = 199). r indicates pearson correlation coefficient, dotted line indicate 95% con fidence interval

Article Snippet: S1P and internal standard were detected using multiple reaction monitoring (m/z 380 → 264 and 366 → 250, respectively), and were quantified using a seven-point calibration curve with d18:1-S1P as external standard (#860,492, Avanti Polar Lipids).

Techniques: Clinical Proteomics

Journal: Cardiovascular diabetology

Article Title: Inverse relationship between circulating sphingosine-1-phosphate and precursor species and coronary artery calcification score in type 2 diabetes.

doi: 10.1186/s12933-025-02624-9

Figure Lengend Snippet: Fig. 4 Clustering of high-density lipoproteins according to the amount of sphingosine-1-phosphate and precursor species. Hierarchical cluster of type 2 diabetic patients based on HDL-S1P species and precursors levels. Three clusters were identified and referred to C1 (n = 26, red), C2 (n = 70, green) and C3 (n = 72, blue) panel A. PCA score panel B. Box plot of HDL-d18:1-S1P levels according to clusters panel C. Variable importance in projection (VIP) score plot panel D. ***p < 0.0005 Cluster 3 vs Cluster 1

Article Snippet: S1P and internal standard were detected using multiple reaction monitoring (m/z 380 → 264 and 366 → 250, respectively), and were quantified using a seven-point calibration curve with d18:1-S1P as external standard (#860,492, Avanti Polar Lipids).

Techniques:

Journal: Cardiovascular diabetology

Article Title: Inverse relationship between circulating sphingosine-1-phosphate and precursor species and coronary artery calcification score in type 2 diabetes.

doi: 10.1186/s12933-025-02624-9

Figure Lengend Snippet: Fig. 5 Clinical and biochemical features of type 2 diabetes patients with high-density lipoproteins impoverished in sphingosine-1-phosphate species. Variations of major biological and biochemical parameters among patients with type 2 diabetes belonging to cluster 3 relative to those belonging to cluster 1 panel A. HDL-S1P levels expressed relative to HDL-cholesterol levels, total S1P panel B, d16:1-S1P (panel C), d18:1-S1P panel D and d18:2-S1P panel E. Cluster 1 (C1, n = 26, red), Cluster 2 (C2, n = 70, green) and Cluster 3, (C3, n = 72, blue). *p < 0.05; **p < 0.005; ***p < 0.0005 Cluster 3 vs Cluster 1

Article Snippet: S1P and internal standard were detected using multiple reaction monitoring (m/z 380 → 264 and 366 → 250, respectively), and were quantified using a seven-point calibration curve with d18:1-S1P as external standard (#860,492, Avanti Polar Lipids).

Techniques: